ELISA/assay

Human Transglutaminase 1, Keratinocyte (TGM1) ELISA Kit

MBS455003

48-Strip-Wells

385 USD

ELISA Kit

Human Transglutaminase 1, Keratinocyte (TGM1) ELISA Kit

[Transglutaminase 1, Keratinocyte (TGM1)]

[ISGF-3; P113; STAT113]

[protein-glutamine gamma-glutamyltransferase K; Protein-glutamine gamma-glutamyltransferase K; protein-glutamine gamma-glutamyltransferase K; transglutaminase 1; Epidermal TGase; Transglutaminase K; TG(K); TGK; TGase K; Transglutaminase-1; TGase-1]

[TGM1]

[TGM1; TGM1; LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE; KTG; TG(K); TGK; TGase K; TGase-1]

Human

817

This assay has high sensitivity and excellent specificity for detection of TGFb3. No significant cross-reactivity or interference between TGFb3 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1000pg/mL. Sensitivity: 6.3pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGFb3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGFb3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TGFb3 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFb3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TGFb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGFb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TGFb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4507475

NP_000350.1

NM_000359.2

P22735

40,628 Da

Developmental Biology Pathway (1270302); Formation Of The Cornified Envelope Pathway (1457791); Keratinization Pathway (1457790)

The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). [provided by RefSeq, Jul 2008]

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation (PubMed:26220141).

48-Strip-Wells

385 USD

96-Strip-Wells

515

5x96-Strip-Wells

2325

10x96-Strip-Wells

4515