ELISA/assay

Human Bactericidal/Permeability Increasing Protein (BPI) ELISA Kit

MBS454952

48-Strip-Wells

385 USD

ELISA Kit

Human Bactericidal/Permeability Increasing Protein (BPI) ELISA Kit

[Bactericidal/Permeability Increasing Protein (BPI)]

[CAP 57]

[bactericidal permeability-increasing protein; Bactericidal permeability-increasing protein; bactericidal permeability-increasing protein; bactericidal/permeability-increasing protein; CAP 57]

[BPI]

[BPI; BPI; rBPI; BPIFD1; BPI]

Human

487

This assay has high sensitivity and excellent specificity for detection of BNP. No significant cross-reactivity or interference between BNP and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.2-2000pg/mL. Sensitivity: 12.42pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of BNP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BNP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

157276599

NP_001716.2

NM_001725.2

P17213

53,900 Da

Antimicrobial Peptides Pathway (1457777); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Neutrophil Degranulation Pathway (1457780); Toll Like Receptor 4 (TLR4) Cascade Pathway (1269234); Toll-Like Receptors Cascades Pathway (1269204)

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has antimicrobial activity against gram-negative organisms. [provided by RefSeq, Nov 2014]

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.

48-Strip-Wells

385 USD

96-Strip-Wells

515

5x96-Strip-Wells

2325

10x96-Strip-Wells

4515