Human HtrA Serine Peptidase 1 (HTRA1) ELISA Kit | MyBioSource MBS454847 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human HtrA Serine Peptidase 1 (HTRA1) ELISA Kit

catalog :

MBS454847

quantity :

48-Strip-Wells

price :

430 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS454847

products type :

ELISA Kit

products full name :

Human HtrA Serine Peptidase 1 (HTRA1) ELISA Kit

products short name :

[HtrA Serine Peptidase 1 (HTRA1)]

products name syn :

[HtrA; L56; ORF480; PRSS11; HtrA Serine Peptidase 1; Protease,Serine,11; IGFBP5-Protease; High-temperature requirement A serine peptidase 1]

other names :

[serine protease HTRA1; Serine protease HTRA1; serine protease HTRA1; HtrA serine peptidase 1; High-temperature requirement A serine peptidase 1; L56; Serine protease 11]

products gene name :

[HTRA1]

other gene names :

[HTRA1; HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2; HTRA; PRSS11]

reactivity :

Human

sequence length :

480

specificity :

This assay has high sensitivity and excellent specificity for detection of HTR7. No significant cross-reactivity or interference between HTR7 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.1-5000pg/mL. Sensitivity: 34.2pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HTR7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HTR7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HTR7 in human serum, plasma, tissue homogenates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTR7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HTR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HTR7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4506141

ncbi acc num :

NP_002766.1

ncbi gb acc num :

NM_002775.4

uniprot acc num :

Q92743

ncbi mol weight :

51,287 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (1270257); Extracellular Matrix Organization Pathway (1270244)

ncbi summary :

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]

uniprot summary :

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

size1 :

48-Strip-Wells

price1 :

430 USD

size2 :

96-Strip-Wells

price2 :

575

size3 :

5x96-Strip-Wells

price3 :

2620

size4 :

10x96-Strip-Wells

price4 :

5095

more info or order :

MyBioSource product webpage