ELISA/assay

Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

MBS454758

48-Strip-Wells

410 USD

ELISA Kit

Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

[N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP)]

[NT-Pro-BNP; N-BNP]

[NT-ProBNP]

Canine

This assay has high sensitivity and excellent specificity for detection of noradrenaline. No significant cross-reactivity or interference between noradrenaline and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 61.7-5,000pg/mL. Sensitivity: 25.5pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level noradrenaline were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level noradrenaline were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of noradrenaline in serum, plasma, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to noradrenaline has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled noradrenaline and unlabeled noradrenaline (Standards or samples) with the pre-coated antibody specific to noradrenaline. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of noradrenaline in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of noradrenaline in the sample.

48-Strip-Wells

410 USD

96-Strip-Wells

545

5x96-Strip-Wells

2480

10x96-Strip-Wells

4820