Human Agrin (AGRN) ELISA Kit | MyBioSource MBS454684 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Agrin (AGRN) ELISA Kit

catalog :

MBS454684

quantity :

48-Strip-Wells

price :

385 USD

product information

catalog number :

MBS454684

products type :

ELISA Kit

products full name :

Human Agrin (AGRN) ELISA Kit

products short name :

[Agrin (AGRN)]

products name syn :

[Agrin Proteoglycan]

other names :

[agrin isoform 2; Agrin; agrin; agrin]

products gene name :

[AGRN]

other gene names :

[AGRN; AGRN; CMS8; CMSPPD; AGRIN; C90; C22]

reactivity :

Human

sequence length :

2045

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 14.6pg/mL

other info2 :

Intra Assay: CV<10%. Inter Assay: CV<12%

ncbi gi num :

54873613

ncbi acc num :

NP_940978.2

ncbi gb acc num :

NM_198576.3

uniprot acc num :

O00468

ncbi mol weight :

216,733 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (1269981); Axon Guidance Pathway (1270303); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1269984); Defective B3GALT6 Causes EDSP2 And SEMDJL1 Pathway (1309217); Defective B3GAT3 Causes JDSSDHD Pathway (1269015); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1269014); Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway (1269021); Defective EXT2 Causes Exostoses 2 Pathway (1269022); Developmental Biology Pathway (1270302); Disease Pathway (1268854)

ncbi summary :

This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

uniprot summary :

Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This secreted isoform forms a bridge, after release from motor neurons, to basal lamina through binding laminin via the NtA domain.Isoform 2: transmembrane form that is the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.Isoform 1, isoform 4 and isoform 5: neuron-specific (z+) isoforms that contain C-terminal insertions of 8-19 AA are potent activators of AChR clustering. Isoform 5, agrin (z+8), containing the 8-AA insert, forms a receptor complex in myotubules containing the neuronal AGRN, the muscle-specific kinase MUSK and LRP4, a member of the LDL receptor family. The splicing factors, NOVA1 and NOVA2, regulate AGRN splicing and production of the 'z' isoforms.Isoform 3 and isoform 6: lack any 'z' insert, are muscle-specific and may be involved in endothelial cell differentiation.Agrin N-terminal 110 kDa subunit: is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling ().

size1 :

48-Strip-Wells

price1 :

385 USD

size2 :

96-Strip-Wells

price2 :

515

size3 :

5x96-Strip-Wells

price3 :

2325

size4 :

10x96-Strip-Wells

price4 :

4515