ELISA/assay

Human Cyclophilin A (CYPA) ELISA Kit

MBS454609

48-Strip-Wells

375 USD

ELISA Kit

Human Cyclophilin A (CYPA) ELISA Kit

[Cyclophilin A (CYPA)]

[AM2]

[peptidyl-prolyl cis-trans isomerase A isoform 2; Peptidyl-prolyl cis-trans isomerase A; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A]

[CYPA]

[PPIA; PPIA; CYPA; CYPH; HEL-S-69p; CYPA; PPIase A]

Human

105

This assay has high sensitivity and excellent specificity for detection of CYPA. No significant cross-reactivity or interference between CYPA and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: <0.124ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYPA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYPA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CYPA in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

665821274

NP_001287910.1

NM_001300981.1

P62937

11,418 Da

APOBEC3G Mediated Resistance To HIV-1 Infection Pathway (1269097); Assembly Of The HIV Virion Pathway (1269086); Basigin Interactions Pathway (1269376); Binding And Entry Of HIV Virion Pathway (1269060); Budding And Maturation Of HIV Virion Pathway (1269090); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Disease Pathway (1268854); Early Phase Of HIV Life Cycle Pathway (1269059); HIV Infection Pathway (1269057); HIV Life Cycle Pathway (1269058)

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

48-Strip-Wells

375 USD

96-Strip-Wells

495

5x96-Strip-Wells

2225

10x96-Strip-Wells

4320