ELISA/assay

Human Semaphorin 3F (SEMA3F) ELISA Kit

MBS454602

48-Strip-Wells

430 USD

ELISA Kit

Human Semaphorin 3F (SEMA3F) ELISA Kit

[Semaphorin 3F (SEMA3F)]

[SEMA-IV; SEMA4; SEMAK; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F]

[semaphorin-3F isoform 2; Semaphorin-3F; semaphorin-3F; semaphorin 3F; Sema III/F; Semaphorin IV; Sema IV]

[SEMA3F]

[SEMA3F; SEMA3F; SEMA4; SEMAK; SEMA-IV; Sema IV]

Human

754

This assay has high sensitivity and excellent specificity for detection of SEMA3E. No significant cross-reactivity or interference between SEMA3E and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.122ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA3E were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA3E were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SEMA3E in mouse serum, plasma, tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA3E. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SEMA3E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA3E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA3E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

974005318

NP_001305729.1

NM_001318800.1

Q13275

85,064 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476)

This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

May play a role in cell motility and cell adhesion.

48-Strip-Wells

430 USD

96-Strip-Wells

575

5x96-Strip-Wells

2620

10x96-Strip-Wells

5095