Human B-Cell Activation Factor Receptor (BAFFR) ELISA Kit | MyBioSource MBS454588 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human B-Cell Activation Factor Receptor (BAFFR) ELISA Kit

catalog :

MBS454588

quantity :

48-Strip-Wells

price :

375 USD

product information

catalog number :

MBS454588

products type :

ELISA Kit

products full name :

Human B-Cell Activation Factor Receptor (BAFFR) ELISA Kit

products short name :

[B-Cell Activation Factor Receptor (BAFFR)]

products name syn :

[CD268; TNFRSF13C; TNFRSF13-C; BAFF-R; BAFFR; BR3; Tumor Necrosis Factor Receptor Superfamily Member 13C]

other names :

[tumor necrosis factor receptor superfamily member 13C; Tumor necrosis factor receptor superfamily member 13C; tumor necrosis factor receptor superfamily member 13C; TNF receptor superfamily member 13C; B-cell-activating factor receptor; BAFF receptor; BAFF-R; BLyS receptor 3; CD_antigen: CD268]

products gene name :

[BAFFR]

other gene names :

[TNFRSF13C; TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin; BAFFR; BR3; BAFF-R]

reactivity :

Human

sequence length :

184

specificity :

This assay has high sensitivity and excellent specificity for detection of BAFFR. No significant cross-reactivity or interference between BAFFR and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.061ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BAFFR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BAFFR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BAFFR in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFFR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BAFFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BAFFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

16445027

ncbi acc num :

NP_443177.1

ncbi gb acc num :

NM_052945.3

uniprot acc num :

Q96RJ3

ncbi mol weight :

18,935 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (1269310); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Immune System Pathway (1269170); Intestinal Immune Network For IgA Production Pathway (128760); Intestinal Immune Network For IgA Production Pathway (128670); NF-kappa B Signaling Pathway (634527); NF-kappa B Signaling Pathway (634652)

ncbi summary :

B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of Baff in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular cysteine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. [provided by RefSeq, Jul 2008]

uniprot summary :

B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.

size1 :

48-Strip-Wells

price1 :

375 USD

size2 :

96-Strip-Wells

price2 :

495

size3 :

5x96-Strip-Wells

price3 :

2225

size4 :

10x96-Strip-Wells

price4 :

4320