catalog number :
MBS454582
products type :
ELISA Kit
products full name :
Human C-Type Lectin Domain Family 3, Member B (CLEC3B) ELISA Kit
products short name :
[C-Type Lectin Domain Family 3, Member B (CLEC3B)]
other names :
[tetranectin isoform 1precursor; Tetranectin; tetranectin; C-type lectin domain family 3 member B; C-type lectin domain family 3 member B; Plasminogen kringle 4-binding protein]
products gene name :
[CLEC3B]
other gene names :
[CLEC3B; CLEC3B; TN; TNA; TNA; TN]
specificity :
This assay has high sensitivity and excellent specificity for detection of CLDN1. No significant cross-reactivity or interference between CLDN1 and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.059ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CLDN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CLDN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CLDN1 in human tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLDN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CLDN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLDN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLDN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_003269.2
ncbi gb acc num :
NM_003278.2
ncbi mol weight :
22,537 Da
ncbi pathways :
Hemostasis Pathway (1269340); Platelet Activation, Signaling And Aggregation Pathway (1269350); Platelet Degranulation Pathway (1269367); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1269365)
uniprot summary :
Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
size4 :
10x96-Strip-Wells