Human Gamma-Glutamyltransferase 1 (gGT1) ELISA Kit | MyBioSource MBS454396 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Gamma-Glutamyltransferase 1 (gGT1) ELISA Kit

catalog :

MBS454396

quantity :

48-Strip-Wells

price :

385 USD

product information

catalog number :

MBS454396

products type :

ELISA Kit

products full name :

Human Gamma-Glutamyltransferase 1 (gGT1) ELISA Kit

products short name :

[Gamma-Glutamyltransferase 1 (gGT1)]

products name syn :

[CD224 G-GT1 GTG Gamma-glutamyltranspeptidase 1 Glutathione hydrolase 1; Leukotriene-C4 hydrolase]

other names :

[gamma-glutamyltranspeptidase 1; Gamma-glutamyltranspeptidase 1; gamma-glutamyltranspeptidase 1; gamma-glutamyltransferase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13]

products gene name :

[gGT1]

other gene names :

[GGT1; GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732; GGT; GGT 1]

reactivity :

Human

sequence length :

569

specificity :

This assay has high sensitivity and excellent specificity for detection of GFAP. No significant cross-reactivity or interference between GFAP and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.134ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GFAP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GFAP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GFAP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GFAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

572152963

ncbi acc num :

NP_001275762.1

ncbi gb acc num :

NM_001288833.1

uniprot acc num :

P19440

ncbi mol weight :

24,080 Da

ncbi pathways :

Aflatoxin Activation And Detoxification Pathway (1270220); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (1270087); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (1270189); Eicosanoid Synthesis Pathway (198888); Glutathione Conjugation Pathway (1270213); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (343); Glutathione Metabolism Pathway (198824)

ncbi summary :

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

uniprot summary :

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide, cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive.

size1 :

48-Strip-Wells

price1 :

385 USD

size2 :

96-Strip-Wells

price2 :

515

size3 :

5x96-Strip-Wells

price3 :

2325

size4 :

10x96-Strip-Wells

price4 :

4515