ELISA/assay

Human Osteocalcin (OC) ELISA Kit

MBS454302

48-Strip-Wells

375 USD

ELISA Kit

Human Osteocalcin (OC) ELISA Kit

[Osteocalcin (OC)]

[BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein]

[osteocalcin preproprotein; Osteocalcin; osteocalcin; bone gamma-carboxyglutamate protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein]

[OC]

[BGLAP; BGLAP; OC; BGP; OCN; BGP]

Human

100

This assay has high sensitivity and excellent specificity for detection of OC. No significant cross-reactivity or interference between OC and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 22.7pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of OC in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of OC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

40316933

NP_954642.1

NM_199173.5

P02818

10,963 Da

FGF Signaling Pathway (137989); Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (1268702); Gamma-carboxylation Of Protein Precursors Pathway (1268704); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (1268703); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); Metabolism Of Proteins Pathway (1268677); Notch-mediated HES/HEY Network Pathway (169347); Osteoblast Signaling Pathway (198842); Post-translational Protein Modification Pathway (1268701)

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

48-Strip-Wells

375 USD

96-Strip-Wells

495

5x96-Strip-Wells

2225

10x96-Strip-Wells

4320