ELISA/assay

Human Deoxyribonuclease I (DNASE1) ELISA Kit

MBS454286

48-Strip-Wells

385 USD

ELISA Kit

Human Deoxyribonuclease I (DNASE1) ELISA Kit

[Deoxyribonuclease I (DNASE1)]

[DNaseI; DNL1; DRNI; DNase-I; Dornase alfa]

[deoxyribonuclease-1; Deoxyribonuclease-1; deoxyribonuclease-1; deoxyribonuclease 1; Deoxyribonuclease I; DNase I; INN: Dornase alfa]

[DNASE1]

[DNASE1; DNASE1; DNL1; DRNI; DNL1; DRNI; DNase I]

Human

282

This assay has high sensitivity and excellent specificity for detection of DNASE1. No significant cross-reactivity or interference between DNASE1 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma, urine, saliva and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.65ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNASE1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNASE1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DNASE1 in human Serum, plasma, urine, saliva and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNASE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DNASE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNASE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DNASE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

21361254

NP_005214.2

NM_005223.3

P24855

19,771 Da

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization ().

48-Strip-Wells

385 USD

96-Strip-Wells

515

5x96-Strip-Wells

2325

10x96-Strip-Wells

4515