Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit | MyBioSource MBS453740 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit

catalog :

MBS453740

quantity :

48-Strip-Wells

price :

355 USD

product information

catalog number :

MBS453740

products type :

ELISA Kit

products full name :

Human Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9) ELISA Kit

products short name :

[Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9)]

products name syn :

[4-1BB-L; Receptor 4-1BB Ligand; Homolog Of Mouse 4-1BB-L]

other names :

[tumor necrosis factor ligand superfamily member 9; Tumor necrosis factor ligand superfamily member 9; tumor necrosis factor ligand superfamily member 9; tumor necrosis factor superfamily member 9; 4-1BB ligand; 4-1BBL]

products gene name :

[TNFSF9]

other gene names :

[TNFSF9; TNFSF9; CD137L; TNLG5A; 4-1BB-L; 4-1BBL]

reactivity :

Human

sequence length :

254

specificity :

This assay has high sensitivity and excellent specificity for detection of TNFSF9. No significant cross-reactivity or interference between TNFSF9 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.62-1000pg/mL. Sensitivity: 5.35pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFSF9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFSF9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFSF9 in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFSF9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TNFSF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFSF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFSF9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4507609

ncbi acc num :

NP_003802.1

ncbi gb acc num :

NM_003811.3

uniprot acc num :

P41273

ncbi mol weight :

26,625 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (1269310); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Factors And Pathways Affecting Insulin-like Growth Factor (IGF1)-Akt Signaling (1458215); Immune System Pathway (1269170); TNFR2 Non-canonical NF-kB Pathway (1269329); TNFs Bind Their Physiological Receptors Pathway (1269332)

ncbi summary :

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008]

uniprot summary :

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

480

size3 :

5x96-Strip-Wells

price3 :

2140

size4 :

10x96-Strip-Wells

price4 :

4155