Mouse Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit | MyBioSource MBS453700 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

catalog :

MBS453700

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS453700

products type :

ELISA Kit

products full name :

Mouse Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

products short name :

[Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)]

products name syn :

[Trem2a; Trem2b; Trem2c; Triggering receptor expressed on monocytes 2]

other names :

[triggering receptor expressed on myeloid cells 2 isoform 2; Triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; Triggering receptor expressed on monocytes 2]

products gene name :

[TREM2]

other gene names :

[Trem2; Trem2; TREM-2; Trem2a; Trem2b; Trem2c; Trem2a; Trem2b; Trem2c; TREM-2]

reactivity :

Mouse

sequence length :

249

specificity :

This assay has high sensitivity and excellent specificity for detection of TPT1. No significant cross-reactivity or interference between TPT1 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Tissue Homogenates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.113ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TPT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TPT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TPT1 in mouse tissue homogenates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TPT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TPT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TPT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

440918700

ncbi acc num :

NP_001259007.1

ncbi gb acc num :

NM_001272078.1

uniprot acc num :

Q99NH8

ncbi mol weight :

27,304 Da

ncbi pathways :

Adaptive Immune System Pathway (1367473); Axon Guidance Pathway (1366709); DAP12 Interactions Pathway (1367572); DAP12 Signaling Pathway (1367573); Developmental Biology Pathway (1366708); Immune System Pathway (1367472); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1367500); Innate Immune System Pathway (1367502); Microglia Pathogen Phagocytosis Pathway (1458488); Osteoclast Differentiation Pathway (193149)

ncbi summary :

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

uniprot summary :

May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

545

size3 :

5x96-Strip-Wells

price3 :

2480

size4 :

10x96-Strip-Wells

price4 :

4820

more info or order :

MyBioSource product webpage