ELISA/assay

Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit

MBS453691

48-Strip-Wells

400 USD

ELISA Kit

Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit

[Na-K-Cl Cotransporter 2 (NKCC2)]

[SLC12A1; NKCC2; Solute Carrier Family 12 Member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter]

[solute carrier family 12 member 1 isoform A; Solute carrier family 12 member 1; solute carrier family 12 member 1; solute carrier family 12 member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter]

[NKCC2]

[SLC12A1; SLC12A1; BSC1; NKCC2; NKCC2]

Human

1099

This assay has high sensitivity and excellent specificity for detection of NGF. No significant cross-reactivity or interference between NGF and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1000pg/mL. Sensitivity: 6.8pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NGF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NGF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NGF in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

134254459

NP_000329.2

NM_000338.2

Q13621

– Da

Cation-coupled Chloride Cotransporters Pathway (1269912); SLC-mediated Transmembrane Transport Pathway (1269907); Transmembrane Transport Of Small Molecules Pathway (1269903); Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway (1269908)

This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]

Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.

48-Strip-Wells

400 USD

96-Strip-Wells

535

5x96-Strip-Wells

2425

10x96-Strip-Wells

4710