Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit | MyBioSource MBS453652 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit

catalog :

MBS453652

quantity :

48-Strip-Wells

price :

400 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS453652

products type :

ELISA Kit

products full name :

Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit

products short name :

[Dual Specificity Phosphatase 1 (DUSP1)]

products name syn :

[CL100; HVH1; MKP1; PTPN10; Mitogen-activated protein kinase phosphatase 1; Protein-tyrosine phosphatase CL100; Dual specificity protein phosphatase hVH1]

other names :

[dual specificity protein phosphatase 1; Dual specificity protein phosphatase 1; dual specificity protein phosphatase 1; dual specificity phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100]

products gene name :

[DUSP1]

other gene names :

[DUSP1; DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10; CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1]

reactivity :

Human

sequence length :

367

specificity :

This assay has high sensitivity and excellent specificity for detection of DNMT3B. No significant cross-reactivity or interference between DNMT3B and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Tissue Homogenates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.054ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNMT3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNMT3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of DNMT3B in human tissue homogenates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNMT3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DNMT3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNMT3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DNMT3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4758204

ncbi acc num :

NP_004408.1

ncbi gb acc num :

NM_004417.3

uniprot acc num :

P28562

ncbi mol weight :

39,298 Da

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); ATF-2 Transcription Factor Network Pathway (138006); Axon Guidance Pathway (1270303); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Developmental Biology Pathway (1270302); Direct P53 Effectors Pathway (137939); Downstream Signal Transduction Pathway (1269479); ErbB1 Downstream Signaling Pathway (138057)

ncbi summary :

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

uniprot summary :

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2425

size4 :

10x96-Strip-Wells

price4 :

4710

more info or order :

MyBioSource product webpage