Human Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) ELISA Kit | MyBioSource MBS453571 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) ELISA Kit

catalog :

MBS453571

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS453571

products type :

ELISA Kit

products full name :

Human Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) ELISA Kit

products short name :

[Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7)]

products name syn :

[CD319; CRACC; 19A; CS1; CD2-like receptor-activating cytotoxic cells; Membrane protein FOAP-12]

other names :

[SLAM family member 7 isoform b; SLAM family member 7; SLAM family member 7; SLAM family member 7; CD2 subset 1; CD2-like receptor-activating cytotoxic cells; CRACC; Membrane protein FOAP-12; Novel Ly9; Protein 19A; CD_antigen: CD319]

products gene name :

[SLAMF7]

other gene names :

[SLAMF7; SLAMF7; 19A; CS1; CD319; CRACC; CS1; CRACC]

reactivity :

Human

sequence length :

165

specificity :

This assay has high sensitivity and excellent specificity for detection of sIgA. No significant cross-reactivity or interference between sIgA and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Serum, Plasma, Tissue Homogenates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.061ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level sIgA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level sIgA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of sIgA in mouse serum, plasma, tissue homogenates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sIgA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to sIgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sIgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of sIgA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

543583691

ncbi acc num :

NP_001269517.1

ncbi gb acc num :

NM_001282588.1

uniprot acc num :

Q9NQ25

ncbi mol weight :

21,316 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Immune System Pathway (1269170); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1269201)

uniprot summary :

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528).

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

480

size3 :

5x96-Strip-Wells

price3 :

2140

size4 :

10x96-Strip-Wells

price4 :

4155

more info or order :

MyBioSource product webpage