Human FK506 Binding Protein 5 (FKBP5) ELISA Kit | MyBioSource MBS453509 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human FK506 Binding Protein 5 (FKBP5) ELISA Kit

catalog :

MBS453509

quantity :

48-Strip-Wells

price :

400 USD

product information

catalog number :

MBS453509

products type :

ELISA Kit

products full name :

Human FK506 Binding Protein 5 (FKBP5) ELISA Kit

products short name :

[FK506 Binding Protein 5 (FKBP5)]

products name syn :

[P54 FKBP51 FKBP54 Ptg-10 Rotamase 51 kDa FK506-binding protein 54 kDa progesterone receptor-associated Androgen-regulated protein 6 HSP90-binding immunophilin]

other names :

[peptidyl-prolyl cis-trans isomerase FKBP5 isoform 1; Peptidyl-prolyl cis-trans isomerase FKBP5; peptidyl-prolyl cis-trans isomerase FKBP5; FK506 binding protein 5; 51 kDa FK506-binding protein; 51 kDa FKBP; FKBP-51; 54 kDa progesterone receptor-associated immunophilin; Androgen-regulated protein 6; FF1 antigen; FK506-binding protein 5; FKBP-5; FKBP54; p54; HSP90-binding immunophilin; Rotamase]

products gene name :

[FKBP5]

other gene names :

[FKBP5; FKBP5; P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10; AIG6; FKBP51; PPIase FKBP5; 51 kDa FKBP; FKBP-51; FKBP-5; p54]

reactivity :

Human

sequence length :

457

specificity :

This assay has high sensitivity and excellent specificity for detection of FKBP5. No significant cross-reactivity or interference between FKBP5 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.061ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FKBP5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FKBP5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FKBP5 in human serum, plasma, tissue homogenates, and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FKBP5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FKBP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FKBP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FKBP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

224809327

ncbi acc num :

NP_001139247.1

ncbi gb acc num :

NM_001145775.2

uniprot acc num :

Q13451

ncbi mol weight :

30,032 Da

ncbi pathways :

Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197); Farnesoid X Receptor Pathway (1458293); Glucocorticoid Receptor Regulatory Network Pathway (138014); MECP2 And Associated Rett Syndrome Pathway (1458229)

ncbi summary :

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

uniprot summary :

Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded.

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2425

size4 :

10x96-Strip-Wells

price4 :

4710