Mouse Apolipoprotein A1 (APOA1) ELISA Kit | MyBioSource MBS453484 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Apolipoprotein A1 (APOA1) ELISA Kit

catalog :

MBS453484

quantity :

48-Strip-Wells

price :

350 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS453484

products type :

ELISA Kit

products full name :

Mouse Apolipoprotein A1 (APOA1) ELISA Kit

products short name :

[Apolipoprotein A1 (APOA1)]

products name syn :

[Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I]

other names :

[apolipoprotein A-I preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apolipoprotein A-I; Apolipoprotein A1]

products gene name :

[APOA1]

other gene names :

[Apoa1; Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I; Apo-AI; ApoA-I; ProapoA-I]

reactivity :

Mouse

sequence length :

264

specificity :

This assay has high sensitivity and excellent specificity for detection of APOA1. No significant cross-reactivity or interference between APOA1 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.67ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APOA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APOA1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of APOA1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APOA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APOA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APOA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APOA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

160333304

ncbi acc num :

NP_033822.2

ncbi gb acc num :

NM_009692.4

uniprot acc num :

Q00623

ncbi mol weight :

30,616 Da

ncbi pathways :

ABC Transporters In Lipid Homeostasis Pathway (1368002); ABC-family Proteins Mediated Transport Pathway (1368001); African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1367201); Chylomicron-mediated Lipid Transport Pathway (1367662); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); HDL-mediated Lipid Transport Pathway (1367663); Hemostasis Pathway (1368135)

ncbi summary :

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013]

uniprot summary :

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

size1 :

48-Strip-Wells

price1 :

350 USD

size2 :

96-Strip-Wells

price2 :

465

size3 :

5x96-Strip-Wells

price3 :

2075

size4 :

10x96-Strip-Wells

price4 :

4020

more info or order :

MyBioSource product webpage