ELISA/assay

Rat Anti-Mullerian Hormone (AMH) ELISA Kit

MBS453381

48-Strip-Wells

395 USD

ELISA Kit

Rat Anti-Mullerian Hormone (AMH) ELISA Kit

[Anti-Mullerian Hormone (AMH)]

[MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance]

[muellerian-inhibiting factor; Muellerian-inhibiting factor; muellerian-inhibiting factor; anti-Mullerian hormone; Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS]

[AMH]

[Amh; Amh; MIS; AMH; MIS]

Rat

553

This assay has high sensitivity and excellent specificity for detection of ALT. No significant cross-reactivity or interference between ALT and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.057ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ALT in mouse serum, plasma, tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

25742760

NP_037034.1

NM_012902.1

P49000

58,889 Da

BMP Signaling Pathway (1085044); BMP Signaling Pathway (1108218); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Hippo Signaling Pathway (749790); Hippo Signaling Pathway (750388); TGF-beta Signaling Pathway (83456); TGF-beta Signaling Pathway (475); CAMP Signaling Pathway (1017647); CAMP Signaling Pathway (1019520)

ligand for MIS receptor II that suppresses female primary and secondary sexual development and allows male sexual differentiation to occur [RGD, Feb 2006]

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.

48-Strip-Wells

395 USD

96-Strip-Wells

525

5x96-Strip-Wells

2380

10x96-Strip-Wells

4620