catalog number :
MBS453271
products type :
ELISA Kit
products full name :
Human Growth Differentiation Factor 11 (GDF11) ELISA Kit
products short name :
[Growth Differentiation Factor 11 (GDF11)]
products name syn :
[Histidine-proline-rich glycoprotein,Histidine-rich glycoprotein,HPRG]
other names :
[growth/differentiation factor 11 preproprotein; Growth/differentiation factor 11; growth/differentiation factor 11; growth differentiation factor 11; Bone morphogenetic protein 11; BMP-11]
products gene name :
[GDF11]
other gene names :
[GDF11; GDF11; BMP11; BMP-11; BMP11; GDF-11; BMP-11]
specificity :
This assay has high sensitivity and excellent specificity for detection of GALT. No significant cross-reactivity or interference between GALT and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.1-5000pg/mL. Sensitivity: 34.5pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GALT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GALT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GALT in human tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GALT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GALT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_005802.1
ncbi gb acc num :
NM_005811.4
ncbi mol weight :
45,091 Da
ncbi summary :
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein plays a role in the development of the nervous and other organ systems, and may regulate aging. [provided by RefSeq, Aug 2016]
uniprot summary :
Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern.
size4 :
10x96-Strip-Wells