catalog number :
MBS453115
products type :
ELISA Kit
products full name :
Mouse Arginase (Arg) ELISA Kit
products short name :
[Arginase (Arg)]
products name syn :
[ARG1; Arginase I; Liver Arginase]
other names :
[arginase-1; Arginase-1; arginase-1; arginase, liver; Liver-type arginase; Type I arginase]
products gene name :
[Arg]
other gene names :
[Arg1; Arg1; AI; PGIF; Arg-1; AI256583]
specificity :
This assay has high sensitivity and excellent specificity for detection of Arg. No significant cross-reactivity or interference between Arg and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.067ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arg were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arg were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Arg in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arg. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Arg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_031508.1
ncbi gb acc num :
NM_007482.3
ncbi mol weight :
34,808 Da
ncbi pathways :
Amino Acid Metabolism Pathway (198416); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (83157); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (83143); Arginine Biosynthesis Pathway (306); Biosynthesis Of Amino Acids Pathway (790952); Biosynthesis Of Amino Acids Pathway (795174); Immune System Pathway (1367472)
size4 :
10x96-Strip-Wells