ELISA/assay

Human Interleukin 17D (IL17D) ELISA Kit

MBS453023

48-Strip-Wells

355 USD

ELISA Kit

Human Interleukin 17D (IL17D) ELISA Kit

[Interleukin 17D (IL17D)]

[Interleukin-27]

[interleukin-17D; Interleukin-17D; interleukin-17D; interleukin 17D; Interleukin-27]

[IL17D]

[IL17D; IL17D; IL-17D; IL-27]

Human

202

This assay has high sensitivity and excellent specificity for detection of IL13. No significant cross-reactivity or interference between IL13 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Bovine Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.2-2000pg/ml. Sensitivity: 13.4pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL13 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL13 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL13 in bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

19923715

NP_612141.1

NM_138284.1

Q8TAD2

21,893 Da

IL17 Signaling Pathway (920968); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Th17 Cell Differentiation Pathway (1469482); Th17 Cell Differentiation Pathway (1469468)

The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent. [provided by RefSeq, Jul 2008]

Induces expression of IL6, CXCL8/IL8, and CSF2/GM-CSF from endothelial cells.

48-Strip-Wells

355 USD

96-Strip-Wells

480

5x96-Strip-Wells

2140

10x96-Strip-Wells

4155