catalog number :
MBS452979
products type :
ELISA Kit
products full name :
General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit
products short name :
[Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)]
products name syn :
[MTLRP; Growth Hormone-Releasing Peptide]
products gene name :
[NAADP]
specificity :
This assay has high sensitivity and excellent specificity for detection of MOTS-c. No significant cross-reactivity or interference between MOTS-c and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
other info1 :
Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 246.9-20,000pg/mL. Sensitivity: 90.3pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MOTS-c were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MOTS-c were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MOTS-c in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to MOTS-c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MOTS-c and unlabeled MOTS-c (Standards or samples) with the pre-coated antibody specific to MOTS-c. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of MOTS-c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of MOTS-c in the sample.
size4 :
10x96-Strip-Wells