ELISA/assay

Mouse Follicle Stimulating Hormone (FSH) ELISA Kit

MBS452569

48-Strip-Wells

380 USD

ELISA Kit

Mouse Follicle Stimulating Hormone (FSH) ELISA Kit

[Follicle Stimulating Hormone (FSH)]

[follitropin subunit beta; Follitropin subunit beta; follitropin subunit beta; follicle stimulating hormone beta; Follicle-stimulating hormone beta subunit; FSH-B; FSH-beta; Follitropin beta chain]

[FSH]

[Fshb; Fshb; FSH; FSH-B; Fshbeta; FSH-beta; FSH-B; FSH-beta]

Mouse

130

This assay has high sensitivity and excellent specificity for detection of FSH. No significant cross-reactivity or interference between FSH and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive inhibition. Detection Range: 3.125-200ng/mL. Sensitivity: 1.49ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FSH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FSH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FSH in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to FSH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FSH and unlabeled FSH (Standards or samples) with the pre-coated antibody specific to FSH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FSH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FSH in the sample.

6679865

NP_032071.1

NM_008045.3

Q60687

14,919 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1367055); G Alpha (s) Signalling Events Pathway (1367085); GPCR Downstream Signaling Pathway (1367084); GPCR Ligand Binding Pathway (1367054); Glycoprotein Hormones Pathway (1367282); GnRH Signaling Pathway (83288); GnRH Signaling Pathway (502); Hormone Ligand-binding Receptors Pathway (1367069); Metabolism Of Proteins Pathway (1367207); Neuroactive Ligand-receptor Interaction Pathway (83250)

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. [provided by RefSeq, Aug 2015]

Stimulates development of follicle and spermatogenesis in the reproductive organs.

48-Strip-Wells

380 USD

96-Strip-Wells

505

5x96-Strip-Wells

2275

10x96-Strip-Wells

4420