Human Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit | MyBioSource MBS452262 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit

catalog :

MBS452262

quantity :

48-Strip-Wells

more info or order :

MyBioSource product webpage

citations: 1

Reference
Wenzel S, Tyurina Y, Zhao J, St Croix C, Dar H, Mao G, et al. PEBP1 Wardens Ferroptosis by Enabling Lipoxygenase Generation of Lipid Death Signals. Cell. 2017;171:628-641.e26 pubmed publisher

image

image 1 :

product information

catalog number :

MBS452262

products type :

ELISA Kit

products full name :

Human Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit

products short name :

[Phosphatidylethanolamine Binding Protein 1 (PEBP1)]

products name syn :

[PBP; HCNP; PEBP; RKIP; Prostatic Binding Protein; Raf Kinase Inhibitory Protein; Hippocampal Cholinergic Neurostimulating Peptide; Neuropolypeptide h3]

other names :

[phosphatidylethanolamine-binding protein 1; Phosphatidylethanolamine-binding protein 1; phosphatidylethanolamine-binding protein 1; phosphatidylethanolamine binding protein 1; HCNPpp; Neuropolypeptide h3; Prostatic-binding protein; Raf kinase inhibitor protein; RKIP]

products gene name :

[PEBP1]

other gene names :

[PEBP1; PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96; PBP; PEBP; PEBP-1; RKIP; HCNP]

reactivity :

Human

sequence length :

187

specificity :

This assay has high sensitivity and excellent specificity for detection of PDCD6IP. No significant cross-reactivity or interference between PDCD6IP and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.107ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDCD6IP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDCD6IP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDCD6IP in human tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDCD6IP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDCD6IP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDCD6IP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDCD6IP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4505621

ncbi acc num :

NP_002558.1

ncbi gb acc num :

NM_002567.3

uniprot acc num :

P30086

ncbi mol weight :

21,057 Da

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Aurora B Signaling Pathway (138080); Axon Guidance Pathway (1270303); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Developmental Biology Pathway (1270302); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855); Downstream Signal Transduction Pathway (1269479)

ncbi summary :

This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]

uniprot summary :

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.

size1 :

48-Strip-Wells

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2425

size4 :

10x96-Strip-Wells

price4 :

4710

more info or order :

MyBioSource product webpage