Human Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit | MyBioSource MBS452204 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

catalog :

MBS452204

quantity :

48-Strip-Wells

price :

400 USD

product information

catalog number :

MBS452204

products type :

ELISA Kit

products full name :

Human Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

products short name :

[Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)]

other names :

[triggering receptor expressed on myeloid cells 2 isoform 2; Triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; Triggering receptor expressed on monocytes 2]

products gene name :

[TREM2]

other gene names :

[TREM2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2]

reactivity :

Human

sequence length :

219

specificity :

This assay has high sensitivity and excellent specificity for detection of TREM2. No significant cross-reactivity or interference between TREM2 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 21.9pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TREM2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TREM2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TREM2 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TREM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TREM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TREM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TREM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

425876764

ncbi acc num :

NP_001258750.1

ncbi gb acc num :

NM_001271821.1

uniprot acc num :

Q9NZC2

ncbi mol weight :

24,669 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Axon Guidance Pathway (1270303); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Developmental Biology Pathway (1270302); Immune System Pathway (1269170); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1269201); Innate Immune System Pathway (1269203); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096)

ncbi summary :

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

uniprot summary :

May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2425

size4 :

10x96-Strip-Wells

price4 :

4710