ELISA/assay

Human Complement Component 1, S Subcomponent (C1s) ELISA Kit

MBS452171

48-Strip-Wells

385 USD

ELISA Kit

Human Complement Component 1, S Subcomponent (C1s) ELISA Kit

[Complement Component 1, S Subcomponent (C1s)]

[C1 esterase]

[complement C1s subcomponent isoform 1 preproprotein; Complement C1s subcomponent; complement C1s subcomponent; complement C1s; C1 esterase; Complement component 1 subcomponent s]

[C1s]

[C1S; C1S; EDSPD2]

Human

688

This assay has high sensitivity and excellent specificity for detection of C1QTNF9. No significant cross-reactivity or interference between C1QTNF9 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12-200ng/mL. Sensitivity: 1.32ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C1QTNF9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C1QTNF9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of C1QTNF9 in human serum, plasma or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1QTNF9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to C1QTNF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C1QTNF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C1QTNF9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4502495

NP_001725.1

NM_001734.4

P09871

76,684 Da

Classical Antibody-mediated Complement Activation Pathway (1269246); Complement Activation Pathway (198823); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1269241); Creation Of C4 And C2 Activators Pathway (1269243); Immune System Pathway (1269170); Initial Triggering Of Complement Pathway (1269242); Innate Immune System Pathway (1269203)

This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq, Mar 2009]

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.

48-Strip-Wells

385 USD

96-Strip-Wells

515

5x96-Strip-Wells

2325

10x96-Strip-Wells

4515