Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit | MyBioSource MBS452015 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit

catalog :

MBS452015

quantity :

48-Strip-Wells

price :

400 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS452015

products type :

ELISA Kit

products full name :

Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit

products short name :

[Doublecortin Like Kinase 1 (DCLK1)]

products name syn :

[DCDC3A; DCLK; DCAMKL1; Doublecortin And CaM Kinase-Like 1; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1]

other names :

[serine/threonine-protein kinase DCLK1 isoform 2; Serine/threonine-protein kinase DCLK1; serine/threonine-protein kinase DCLK1; doublecortin like kinase 1; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1]

products gene name :

[DCLK1]

other gene names :

[DCLK1; DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1; DCAMKL1; DCDC3A; KIAA0369]

reactivity :

Human

sequence length :

422

specificity :

This assay has high sensitivity and excellent specificity for detection of CYP2E1. No significant cross-reactivity or interference between CYP2E1 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12-200ng/mL. Sensitivity: 1.29ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP2E1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP2E1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CYP2E1 in mouse tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP2E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYP2E1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYP2E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

306518604

ncbi acc num :

NP_001182344.1

ncbi gb acc num :

NM_001195415.1

uniprot acc num :

O15075

ncbi mol weight :

47,681 Da

ncbi summary :

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]

uniprot summary :

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2425

size4 :

10x96-Strip-Wells

price4 :

4710

more info or order :

MyBioSource product webpage