Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit | MyBioSource MBS451995 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

catalog :

MBS451995

quantity :

48-Strip-Wells

price :

345 USD

product information

catalog number :

MBS451995

products type :

ELISA Kit

products full name :

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

products short name :

[Fibroblast Growth Factor 10 (FGF10)]

products name syn :

[Keratinocyte growth factor 2]

other names :

[fibroblast growth factor 10; Fibroblast growth factor 10; fibroblast growth factor 10; fibroblast growth factor 10; Keratinocyte growth factor 2]

products gene name :

[FGF10]

other gene names :

[FGF10; FGF10; FGF-10]

reactivity :

Human

sequence length :

208

specificity :

This assay has high sensitivity and excellent specificity for detection of FFAR2. No significant cross-reactivity or interference between FFAR2 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.055ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FFAR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FFAR2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FFAR2 in mouse tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FFAR2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FFAR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FFAR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FFAR2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4758360

ncbi acc num :

NP_004456.1

ncbi gb acc num :

NM_004465.1

uniprot acc num :

O15520

ncbi mol weight :

23,436 Da

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Activated Point Mutants Of FGFR2 Pathway (1268871); Adaptive Immune System Pathway (1269171); Axon Guidance Pathway (1270303); Breast Cancer Pathway (1435207); Breast Cancer Pathway (1435182); Constitutive Signaling By Aberrant PI3K In Cancer Pathway (1268880); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]

uniprot summary :

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.

size1 :

48-Strip-Wells

price1 :

345 USD

size2 :

96-Strip-Wells

price2 :

460

size3 :

5x96-Strip-Wells

price3 :

2045

size4 :

10x96-Strip-Wells

price4 :

3960