ELISA/assay

Human Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit

MBS451986

48-Strip-Wells

400 USD

ELISA Kit

Human Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit

[Cathelicidin Antimicrobial Peptide (CAMP)]

[CAP18 FALL39 HSD26 LL37 18 kDa Cationic Antimicrobial Protein Antibacterial protein FALL-39 FALL-39 peptide antibiotic]

[cathelicidin antimicrobial peptide preproprotein; Cathelicidin antimicrobial peptide; cathelicidin antimicrobial peptide; cathelicidin antimicrobial peptide; FALL-39 peptide antibiotic]

[CAMP]

[CAMP; CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39; hCAP-18]

Human

173

This assay has high sensitivity and excellent specificity for detection of C3. No significant cross-reactivity or interference between C3 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.061ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of C3 in human serum, plasma or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

348041314

NP_004336.3

NM_004345.4

P49913

19,301 Da

Antimicrobial Peptides Pathway (1457777); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Integrated Pancreatic Cancer Pathway (711360); NOD-like Receptor Signaling Pathway (122191); NOD-like Receptor Signaling Pathway (122131); Neutrophil Degranulation Pathway (1457780); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Sterol Regulatory Element-Binding Proteins (SREBP) Signalling Pathway (219802)

This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]

Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.

48-Strip-Wells

400 USD

96-Strip-Wells

535

5x96-Strip-Wells

2425

10x96-Strip-Wells

4710