Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit | MyBioSource MBS451944 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit

catalog :

MBS451944

quantity :

48-Strip-Wells

price :

350 USD

product information

catalog number :

MBS451944

products type :

ELISA Kit

products full name :

Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit

products short name :

[Fatty Acid Binding Protein 2, Intestinal (FABP2)]

products name syn :

[FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein]

other names :

[fatty acid-binding protein, intestinal; Fatty acid-binding protein, intestinal; fatty acid-binding protein, intestinal; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP]

products gene name :

[FABP2]

other gene names :

[Fabp2; Fabp2; Fabpi; I-FABP; Fabpi; I-FABP]

reactivity :

Mouse

sequence length :

132

specificity :

This assay has high sensitivity and excellent specificity for detection of ETRA. No significant cross-reactivity or interference between ETRA and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Rat Serum, Plasma, Tissue Homogenates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.040g/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ETRA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ETRA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ETRA in rat serum, plasma, tissue homogenates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ETRA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ETRA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ETRA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ETRA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6679737

ncbi acc num :

NP_032006.1

ncbi gb acc num :

NM_007980.3

uniprot acc num :

P55050

ncbi mol weight :

15,126 Da

ncbi pathways :

Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway (1367665); Lipid Digestion, Mobilization, And Transport Pathway (1367658); Metabolism Pathway (1367614); Metabolism Of Lipids And Lipoproteins Pathway (1367657); PPAR Signaling Pathway (83239); PPAR Signaling Pathway (450); PPAR Signaling Pathway (694605)

ncbi summary :

The protein encoded by this gene is part of the fatty acid binding protein family (FABP). FABPs are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands and participate in fatty acid uptake, transport, and metabolism. This protein functions within enterocytes, possibly to sense lipids as part of energy homeostasis. In humans polymorphisms are associated with increased fat oxidation and insulin resistance. In mice deficiency of this gene alters body weight in a gender-specific manner and causes hyperinsulinemia. [provided by RefSeq, Jan 2013]

uniprot summary :

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

size1 :

48-Strip-Wells

price1 :

350 USD

size2 :

96-Strip-Wells

price2 :

465

size3 :

5x96-Strip-Wells

price3 :

2075

size4 :

10x96-Strip-Wells

price4 :

4020