ELISA/assay

Rat Osteocalcin (OC) ELISA Kit

MBS451886

48-Strip-Wells

395 USD

ELISA Kit

Rat Osteocalcin (OC) ELISA Kit

[Osteocalcin (OC)]

[BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein]

[osteocalcin preproprotein; Osteocalcin; osteocalcin; bone gamma-carboxyglutamate protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein]

[OC]

[Bglap; Bglap; Bgp; Bgpr; Bgpra; Bglap2; Bglap2; BGP]

Rat

99

This assay has high sensitivity and excellent specificity for detection of OC. No significant cross-reactivity or interference between OC and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 23.58pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of OC in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of OC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

11761543

NP_038200.1

NM_013414.1

P04640

10,927 Da

Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (1377487); Gamma-carboxylation Of Protein Precursors Pathway (1377489); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (1377488); Metabolism Of Proteins Pathway (1377481); Osteoblast Pathway (198455); Post-translational Protein Modification Pathway (1377486); Removal Of Aminoterminal Propeptides From Gamma-carboxylated Proteins Pathway (1377491); Transport Of Gamma-carboxylated Protein Precursors From The Endoplasmic Reticulum To The Golgi Apparatus Pathway (1377490)

highly conserved protein associated with mineralized bone matrix; protein is secreted by calcified tissues and is regulated by vitamin D3 [RGD, Feb 2006]

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

48-Strip-Wells

395 USD

96-Strip-Wells

525

5x96-Strip-Wells

2380

10x96-Strip-Wells

4620