Mouse Adiponectin (ADP) ELISA Kit | MyBioSource MBS451838 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Adiponectin (ADP) ELISA Kit

catalog :

MBS451838

quantity :

48-Strip-Wells

price :

260 USD

product information

catalog number :

MBS451838

products type :

ELISA Kit

products full name :

Mouse Adiponectin (ADP) ELISA Kit

products short name :

[Adiponectin (ADP)]

products name syn :

[GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1]

other names :

[adiponectin; Adiponectin; adiponectin; adiponectin, C1Q and collagen domain containing; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ]

products gene name :

[ADP]

other gene names :

[Adipoq; Adipoq; Ad; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30; Acdc; Acrp30; Apm1; ACRP30]

reactivity :

Mouse

sequence length :

247

specificity :

This assay has high sensitivity and excellent specificity for detection of ADP. No significant cross-reactivity or interference between ADP and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 14.5pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

87252711

ncbi acc num :

NP_033735.3

ncbi gb acc num :

NM_009605.5

uniprot acc num :

Q60994

ncbi mol weight :

26,809 Da

ncbi pathways :

AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway (1367767); AMPK Signaling Pathway (989651); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Adipogenesis Genes Pathway (198299); Integration Of Energy Metabolism Pathway (1367756); Leptin And Adiponectin Pathway (198344); Longevity Regulating Pathway (1320038); Metabolism Pathway (1367614)

uniprot summary :

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

size1 :

48-Strip-Wells

price1 :

260 USD

size2 :

96-Strip-Wells

price2 :

320

size3 :

5x96-Strip-Wells

price3 :

1375

size4 :

10x96-Strip-Wells

price4 :

2665