catalog number :
MBS451536
products type :
ELISA Kit
products full name :
Canine Atrial Natriuretic Peptide (ANP) ELISA Kit
products short name :
[Atrial Natriuretic Peptide (ANP)]
products name syn :
[ANH; ANF; PND; NPPA; CDD-ANF; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Natriuretic Peptide Precursor A; Prepronatriodilatin; Cardiodilatin-related peptide]
other names :
[natriuretic peptides A; Natriuretic peptides A; natriuretic peptides A; Prepronatriodilatin]
products gene name :
[ANP]
other gene names :
[NPPA; NPPA; ANF; ANP; ANF; ANP]
specificity :
This assay has high sensitivity and excellent specificity for detection of ANGPTL8. No significant cross-reactivity or interference between ANGPTL8 and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.1-5000pg/mL. Sensitivity: 30pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANGPTL8 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANGPTL8 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANGPTL8 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANGPTL8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ANGPTL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANGPTL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ANGPTL8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_001300780.1
ncbi gb acc num :
NM_001313851.1
ncbi mol weight :
15,819 Da
ncbi pathways :
Cardiac Conduction Pathway (1384107); HIF-1 Signaling Pathway (695209); Muscle Contraction Pathway (1384104); Physiological Factors Pathway (1384114)
uniprot summary :
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 ().
size4 :
10x96-Strip-Wells