Human Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit | MyBioSource MBS451501 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit

catalog :

MBS451501

quantity :

48-Strip-Wells

price :

385 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS451501

products type :

ELISA Kit

products full name :

Human Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit

products short name :

[Low Density Lipoprotein Receptor Related Protein 1 (LRP1)]

products name syn :

[CD91; LRP; A2MR; APOER; APR; TGFBR5; Alpha-2-Macroglobulin Receptor; Prolow-density lipoprotein receptor-related protein 1; Apolipoprotein E receptor]

other names :

[prolow-density lipoprotein receptor-related protein 1 preproprotein; Prolow-density lipoprotein receptor-related protein 1; prolow-density lipoprotein receptor-related protein 1; LDL receptor related protein 1; Alpha-2-macroglobulin receptor; A2MR]

products gene name :

[LRP1]

other gene names :

[LRP1; LRP1; APR; KPA; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R; A2MR; APR; LRP-1; A2MR; APOER; LRP-85; LRP-515; LRPICD]

reactivity :

Human

sequence length :

4544

specificity :

This assay has high sensitivity and excellent specificity for detection of LRP1. No significant cross-reactivity or interference between LRP1 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma or other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: <0.255ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LRP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LRP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LRP1 in human serum, plasma or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LRP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LRP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

126012562

ncbi acc num :

NP_002323.2

ncbi gb acc num :

NM_002332.2

uniprot acc num :

Q07954

ncbi mol weight :

31,631 Da

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1269897); Malaria Pathway (152665); Malaria Pathway (152657); Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147); Metabolism Of Vitamins And Cofactors Pathway (1270144); PDGFR-beta Signaling Pathway (138071)

ncbi summary :

This gene encodes a member of the low-density lipoprotein receptor family of proteins. The encoded preproprotein is proteolytically processed by furin to generate 515 kDa and 85 kDa subunits that form the mature receptor (PMID: 8546712). This receptor is involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the alpha 2-macroglobulin-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer's disease patients. [provided by RefSeq, Oct 2015]

uniprot summary :

Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.

size1 :

48-Strip-Wells

price1 :

385 USD

size2 :

96-Strip-Wells

price2 :

515

size3 :

5x96-Strip-Wells

price3 :

2325

size4 :

10x96-Strip-Wells

price4 :

4515

more info or order :

MyBioSource product webpage