catalog number :
MBS451500
products type :
ELISA Kit
products full name :
Rat N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit
products short name :
[N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP)]
products name syn :
[NT-Pro-BNP; N-BNP]
products gene name :
[NT-ProBNP]
specificity :
This assay has high sensitivity and excellent specificity for detection of NT-ProBNP. No significant cross-reactivity or interference between NT-ProBNP and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Porcine Serum, Plasma Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: 3.32pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NT-ProBNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NT-ProBNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NT-ProBNP in porcine serum, plasma or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NT-ProBNP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NT-ProBNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
size4 :
10x96-Strip-Wells