ELISA/assay

Human 5'-Nucleotidase, Ecto (NT5E) ELISA Kit

MBS451492

48-Strip-Wells

385 USD

ELISA Kit

Human 5'-Nucleotidase, Ecto (NT5E) ELISA Kit

[5'-Nucleotidase, Ecto (NT5E)]

[ALG-2; PEF1B; Apoptosis-Linked Gene-2]

[5'-nucleotidase (Ecto-5'-nucleotidase); 5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase ecto; 5'-nucleotidase]

[NT5E]

[NT5E; NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA]

Human

574

This assay has high sensitivity and excellent specificity for detection of NT5E. No significant cross-reactivity or interference between NT5E and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.66ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NT5E were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NT5E were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NT5E in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NT5E. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NT5E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NT5E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NT5E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

74727144

Q53Z63

63,338 Da

Differentiation Pathway (1458184); HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Nucleotides Pathway (1270133); Metabolism Of Vitamins And Cofactors Pathway (1270144); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (1270145); NAD+ Metabolism Pathway (1458237); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400)

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

48-Strip-Wells

385 USD

96-Strip-Wells

515

5x96-Strip-Wells

2325

10x96-Strip-Wells

4515