Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit | MyBioSource MBS451182 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit

catalog :

MBS451182

quantity :

48-Strip-Wells

price :

410 USD

product information

catalog number :

MBS451182

products type :

ELISA Kit

products full name :

Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit

products short name :

[Regenerating Islet Derived Protein 3 Gamma (REG3g)]

products name syn :

[REG-III; PAP1B; PAPIB; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma]

other names :

[regenerating islet-derived protein 3-gamma preproprotein; Regenerating islet-derived protein 3-gamma; regenerating islet-derived protein 3-gamma; regenerating islet-derived 3 gamma; Pancreatitis-associated protein 3; Regenerating islet-derived protein III-gamma; Reg III-gamma]

products gene name :

[REG3g]

other gene names :

[Reg3g; Reg3g; AI449515; REG-3-gamma; reg III-gamma; Pap3; REG-3-gamma; Reg III-gamma]

reactivity :

Mouse

sequence length :

174

specificity :

This assay has high sensitivity and excellent specificity for detection of REG3g. No significant cross-reactivity or interference between REG3g and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: 6.9pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REG3g were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REG3g were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of REG3g in mouse serum, plasma and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG3g. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to REG3g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG3g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of REG3g in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6755310

ncbi acc num :

NP_035390.1

ncbi gb acc num :

NM_011260.2

uniprot acc num :

O09049

ncbi mol weight :

19,307 Da

ncbi pathways :

Antimicrobial Peptides Pathway (1457936); Immune System Pathway (1367472); Innate Immune System Pathway (1367502)

ncbi summary :

This gene encodes a C-type lectin that demonstrates bactericidal activity. This gene is predominantly expressed in the distal small intestine where the encoded protein undergoes proteolytic processing by trypsin. Mice lacking the encoded protein exhibit altered mucus distribution, increased bacterial contact with the epithelium, and elevated inflammatory markers in the ileum, and low-grade inflammation. [provided by RefSeq, Jun 2016]

uniprot summary :

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

545

size3 :

5x96-Strip-Wells

price3 :

2480

size4 :

10x96-Strip-Wells

price4 :

4820