ELISA/assay

Human Islet Amyloid Polypeptide (IAPP) ELISA Kit

MBS450907

48-Strip-Wells

375 USD

ELISA Kit

Human Islet Amyloid Polypeptide (IAPP) ELISA Kit

[Islet Amyloid Polypeptide (IAPP)]

[IAP; DAP; Amylin; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide]

[islet amyloid polypeptide preproprotein; Islet amyloid polypeptide; islet amyloid polypeptide; islet amyloid polypeptide; Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide]

[IAPP]

[IAPP; IAPP; DAP; IAP; DAP]

Human

89

This assay has high sensitivity and excellent specificity for detection of IAA. No significant cross-reactivity or interference between IAA and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Samples: Tissue Or Cell Culture Supernates. Assay Type: Quantitative Competitive. Detection Range: 320-200,000pg/mL. Sensitivity: 112pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IAA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IAA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IAA in tissue or cell culture supernates. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to IAA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IAA and unlabeled IAA (Standards or samples) with the pre-coated antibody specific to IAA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IAA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IAA in the sample.

4557655

NP_000406.1

NM_000415.2

P10997

9,806 Da

Amyloid Fiber Formation Pathway (1269169); Calcitonin-like Ligand Receptors Pathway (1269571); Class B/2 (Secretin Family Receptors) Pathway (1269570); Developmental Biology Pathway (1270302); G Alpha (s) Signalling Events Pathway (1269575); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Integrated Pancreatic Cancer Pathway (711360); Maturity Onset Diabetes Of The Young Pathway (83096); Maturity Onset Diabetes Of The Young Pathway (508)

This gene encodes a member of the calcitonin family of peptide hormones. This hormone is released from pancreatic beta cells following food intake to regulate blood glucose levels and act as a satiation signal. Human patients with type 1 and advanced type 2 diabetes exhibit reduced levels of the encoded hormone in blood and pancreas. This protein also exhibits a bactericidal, antimicrobial activity. [provided by RefSeq, Jul 2016]

Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.

48-Strip-Wells

375 USD

96-Strip-Wells

495

5x96-Strip-Wells

2225

10x96-Strip-Wells

4320