Human Arginase II (Arg2) ELISA Kit | MyBioSource MBS450779 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Arginase II (Arg2) ELISA Kit

catalog :

MBS450779

quantity :

48-Strip-Wells

price :

400 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS450779

products type :

ELISA Kit

products full name :

Human Arginase II (Arg2) ELISA Kit

products short name :

[Arginase II (Arg2)]

products name syn :

[Kidney-type arginase; Non-hepatic arginase]

other names :

[arginase-2, mitochondrial; Arginase-2, mitochondrial; arginase-2, mitochondrial; arginase 2; Kidney-type arginase; Non-hepatic arginase; Type II arginase]

products gene name :

[Arg2]

other gene names :

[ARG2; ARG2]

reactivity :

Human

sequence length :

354

specificity :

This assay has high sensitivity and excellent specificity for detection of Arg2. No significant cross-reactivity or interference between Arg2 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: 1.15ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arg2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arg2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Arg2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arg2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Arg2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arg2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arg2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4502215

ncbi acc num :

NP_001163.1

ncbi gb acc num :

NM_001172.3

uniprot acc num :

P78540

ncbi mol weight :

38,578 Da

ncbi pathways :

Amino Acid Metabolism Pathway (1471249); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (82943); Arginine Biosynthesis Pathway (306); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); L-arginine Degradation I (arginase Pathway) (138167)

ncbi summary :

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2425

size4 :

10x96-Strip-Wells

price4 :

4710

more info or order :

MyBioSource product webpage