catalog number :
MBS450645
products type :
ELISA Kit
products full name :
Mouse Tryptase (TPS) ELISA Kit
products short name :
[Tryptase (TPS)]
products name syn :
[TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III]
other names :
[tryptase; Tryptase; tryptase; tryptase alpha/beta 1; Mast cell protease 7; mMCP-7; Tryptase alpha/beta-1]
products gene name :
[TPS]
other gene names :
[Tpsab1; Tpsab1; Mcp7; Mcp-7; Mcpt7; MMCP-7; Mcpt7; mMCP-7]
specificity :
This assay has high sensitivity and excellent specificity for detection of TPS. No significant cross-reactivity or interference between TPS and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: 31pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TPS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TPS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TPS in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_112464.4
ncbi gb acc num :
NM_031187.4
ncbi mol weight :
4,896 Da
uniprot summary :
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity ().
size4 :
10x96-Strip-Wells