ELISA/assay

Human Ferroportin (FPN) ELISA Kit

MBS450431

48-Strip-Wells

400 USD

ELISA Kit

Human Ferroportin (FPN) ELISA Kit

[Ferroportin (FPN)]

[solute carrier family 40 member 1; Solute carrier family 40 member 1; solute carrier family 40 member 1; solute carrier family 40 member 1; Ferroportin-1; Iron-regulated transporter 1]

[FPN]

[SLC40A1; SLC40A1; FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3; FPN1; IREG1; SLC11A3]

Human

571

This assay has high sensitivity and excellent specificity for detection of FOXP3. No significant cross-reactivity or interference between FOXP3 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.122ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FOXP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FOXP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FOXP3 in mouse tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FOXP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FOXP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FOXP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FOXP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

7657100

NP_055400.1

NM_014585.5

Q9NP59

62,542 Da

Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (1269948); Metal Ion SLC Transporters Pathway (1269930); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); SLC-mediated Transmembrane Transport Pathway (1269907); Transmembrane Transport Of Small Molecules Pathway (1269903); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (1269923)

The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008]

May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin).

48-Strip-Wells

400 USD

96-Strip-Wells

535

5x96-Strip-Wells

2425

10x96-Strip-Wells

4710