Human Transferrin Receptor (TFR) ELISA Kit | MyBioSource MBS4502796 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Transferrin Receptor (TFR) ELISA Kit

catalog :

MBS4502796

quantity :

48-Strip-Wells

price :

405 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS4502796

products type :

RTU ELISA Kit

products full name :

Human Transferrin Receptor (TFR) ELISA Kit

products short name :

[Transferrin Receptor (TFR)]

products name syn :

[CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form]

other names :

[transferrin receptor protein 1 isoform 1; Transferrin receptor protein 1; transferrin receptor protein 1; transferrin receptor; T9; p90; CD_antigen: CD71]

products gene name :

[TFR]

other gene names :

[TFRC; TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46; TR; TfR; TfR1; Trfr; sTfR]

reactivity :

Human

sequence length :

760

specificity :

This assay has high sensitivity and excellent specificity for detection of TECK. No significant cross-reactivity or interference between TECK and analogues was observed.

storage stability :

For unopened kits: All the reagents should be kept at 4 degree C upon receipt. For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Serum, Plasma, Tissue Homogenates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.2-2000pg/mL. Sensitivity: 11.1pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TECK were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TECK were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TECK in mouse serum, plasma, tissue homogenates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TECK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TECK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TECK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TECK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

189458819

ncbi acc num :

NP_001121620.1

ncbi gb acc num :

NM_001128148.2

uniprot acc num :

P02786

ncbi mol weight :

84,871 Da

ncbi pathways :

Clathrin Derived Vesicle Budding Pathway (119545); Endocytosis Pathway (102279); Endocytosis Pathway (102181); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Golgi Associated Vesicle Biogenesis Pathway (119546); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Iron Metabolism In Placenta Pathway (672461)

ncbi summary :

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

uniprot summary :

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).

size1 :

48-Strip-Wells

price1 :

405 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2430

size4 :

10x96-Strip-Wells

price4 :

4720

more info or order :

MyBioSource product webpage