ELISA/assay

Mouse Vimentin (VIM) ELISA Kit

MBS450175

48-Strip-Wells

395 USD

ELISA Kit

Mouse Vimentin (VIM) ELISA Kit

[Vimentin (VIM)]

[vimentin; Vimentin; vimentin; vimentin]

[VIM]

[Vim; Vim]

Mouse

466

This assay has high sensitivity and excellent specificity for detection of VGF. No significant cross-reactivity or interference between VGF and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.2-2000pg/mL. Sensitivity: 12.4pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VGF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VGF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of VGF in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

31982755

NP_035831.2

NM_011701.4

P20152

53,688 Da

Alpha6-Beta4 Integrin Signaling Pathway (198373); Apoptosis Pathway (1366778); Apoptotic Cleavage Of Cellular Proteins Pathway (1366800); Apoptotic Execution Phase Pathway (1366799); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1366801); Epstein-Barr Virus Infection Pathway (585576); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852715); MicroRNAs In Cancer Pathway (852928); Muscle Contraction Pathway (1366766)

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

48-Strip-Wells

395 USD

96-Strip-Wells

525

5x96-Strip-Wells

2380

10x96-Strip-Wells

4620