ATG12 Polyclonal Antibody | MyBioSource MBS448066 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ATG12 Polyclonal Antibody

catalog :

MBS448066

quantity :

0.6 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

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product information

catalog number :

MBS448066

products type :

Antibody

products full name :

ATG12 Polyclonal Antibody

products short name :

[ATG12]

products name syn :

[Anti-ATG12; APG12; Apg12 (autophagy; yeast) homolog; APG12L; FBR93; HAPG12; autophagy protein 12; autophagy-related protein 12 antibody]

other names :

[Atg12; Ubiquitin-like protein ATG12; ubiquitin-like protein ATG12; Apg12 (autophagy, yeast) homolog; ATG12 autophagy related 12 homolog; autophagy related 12; Autophagy-related protein 12]

products gene name :

[ATG12]

other gene names :

[ATG12; ATG12; APG12; FBR93; APG12L; HAPG12; APG12; APG12L; APG12-like]

uniprot entry name :

ATG12_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

reactivity :

Rat, mouse, canine and monkey proteins.

sequence length :

187

specificity :

Detects GFP-ATG12 in transfected cells by Western blot.

form :

Polyclonal antibody supplied as a 200 uL(3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

storage stability :

Store at 2-8°C for up to one month. Store at -20°C for long term. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence

app notes :

Western Blot: 1:250-1:2,000. Immunofluorescence: 1:50-1:250

image1 heading :

Reactivity Chart

image2 heading :

Testing Data

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.

products description :

Autophagy related 12. Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

ncbi gi num :

189031605

ncbi acc num :

ACD74941.1

uniprot acc num :

O94817

ncbi pathways :

FoxO Signaling Pathway (921162); Immune System Pathway (106386); Innate Immune System Pathway (106387); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (187097); Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780)

ncbi summary :

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

uniprot summary :

Function: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through an ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. Ref.5 Ref.9 Ref.11 Ref.12 Ref.14 Ref.16 Ref.19. Subunit structure: Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3 and TECPR1. Ref.4 Ref.5 Ref.6 Ref.9 Ref.18 Ref.19. Subcellular location: Cytoplasm . By similarity. Preautophagosomal structure membrane; Peripheral membrane protein. Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane. Ref.18. Tissue specificity: Ubiquitous. Ref.1. Induction: Expression is induced by mitochondrial DNA deletions, chloramphenicol and nicotinamide. Ref.8 Ref.13. Domain: Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. Ref.10. Post-translational modification: Acetylated by EP300. Ref.15. Miscellaneous: Small amount of ATG5-ATG12 conjugate is enough to perform normal autophagy. Sequence similarities: Belongs to the ATG12 family. Sequence caution: The sequence AAH12266.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

size1 :

0.6 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage