antibody

Connexin 43 Polyclonal Antibody

MBS448055

0.8 mg

300 USD

polyclonal

goat

nonconjugated

mouse, rat, dog, zebrafish

western blot, immunocytochemistry

Antibody

Connexin 43 Polyclonal Antibody

[CX43]

[Anti-CX43; AVSD3; CX43; DFNB38; gap junction protein; alpha 1; 43kDa; GJA1; GJAL HSS; HLHS1; ODDD antibody]

[Cx43 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; shf; sof; connexin-43; etID309742.9; short fin protein; gap junction protein, alpha 1; connexin 43; Connexin-43; Cx43; Short fin protein]

[CX43]

[cx43; gja1; gja1; cx43a1; zfCx43.3; cx43; shf; sof; Cx43]

CXA1_DANRE

Polyclonal

IgG

Goat

Reacts against human, rat, mouse, canine and monkey proteins

282

Detects endogenous levels of total connexin 43.

Polyclonal antibody supplied as a 200 uL (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Store at -20 C for long term storage. Store at 2-8 C for up to one month. Avoid freeze/thaw cycles.

Immunofluorescence (IF), Western Blot (WB)

Usage:. Western blot: 1:500-1:2,000. Immunofluorescence: 1:50-1:250. Immunohistochemistry (parafin): 1:250-1:800. Immunohistochemistry (frozen): 1:250-1:800

Reactivity Chart

Testing Data

Immunogen: Purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E. coli.

Handling: The antibody solution should be gently mixed before use.

Gap junction protein, alpha 1, 43kDa. Goat polyclonal to connexin 43, which is a member of the connexin family. Cx43 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. This protein is the major protein of gap junctions in embryonic development and in the heart that are thought to have a crucial role in the synchronized contraction of the heart.

29179440

AAH49297.1

O57474

Calcium Regulation In The Cardiac Cell Pathway (198967); EGFR1 Signaling Pathway (198963); Formation Of Annular Gap Junctions Pathway (929228); Gap Junction Pathway (119270); Gap Junction Pathway (483); Gap Junction Assembly Pathway (929222); Gap Junction Degradation Pathway (929227); Gap Junction Trafficking Pathway (929221); Gap Junction Trafficking And Regulation Pathway (929220); Membrane Trafficking Pathway (929211)

Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Ref.1Plays a role in regulation of fin bone size and growth. Ref.1. Subunit structure: A connexon is composed of a hexamer of connexins. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction gap junction. Tissue specificity: Expressed in adult ovary, heart, lens and liver. In growing caudal fins, expressed in cells flanking the germinal region of newly growing segments and in osteoblasts at segment boundaries. Ref.1 Ref.2 Ref.5. Developmental stage: Expressed both maternally and zygotically. Zygotic expression is initiated before gastrulation and persists through to day 5 of development. Levels increase in late somitogenesis, and late embryonic and early larval stages. Embryonic expression is observed in many developing tissues and organs, including the notochord and brain, and heart and vasculature. Detected throughout the lens at 24 hours post-fertilization (hpf), but by 3-4 days development, restricted to the lateral epithelium and differentiating cells in the equatorial region. Ref.2 Ref.5. Disruption phenotype: Morpholino knockdown of the protein results in heart folding defects, reduced hematopoiesis and small eyes. Ref.1. Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.

0.8 mg

300 USD