antibody

ZO-1 Polyclonal Antibody

MBS448025

0.6 mg

410 USD

polyclonal

goat

nonconjugated

mouse, rat, dog

western blot, immunocytochemistry

Antibody

ZO-1 Polyclonal Antibody

ZO-1

Anti-ZO-1; ZO-1 and zona occludens protein 1 antibody

ZO-1; Tight junction protein ZO-1; tight junction protein ZO-1; zona occludens protein 1; zonula occludens protein 1; tight junction protein 1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1

ZO-1

Tjp1; Tjp1; ZO1; Zo1

ZO1_MOUSE

Polyclonal

IgG

Goat

Reacts against human, rat, mouse, canine and monkey proteins.

1745

Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates.

Polyclonal antibody supplied as a 200 ul(3 mg/ml) aliquot in PBS, 20% glycerol and 0.05%

Store at -20 degree C for long-term storage. Store at 2-8 degree C for up to one month. A void freeze/thaw cycles.

Immunofluorescence (IF), Western Blot (WB)

Western blot: 1:500-1:2,000. lmm unofl uorescence: 1:50-1:250. Immunohistochemistry (paraffin): 1:50-1:200. Immunohistochemistry (frozen): 1:50-1:200

Gene Identifier: ENSG00000104067. Immunogen: Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.

Handling: The antibody solution should be gently mixed before use.

Goat polyclonal to TJPl tight Junction protein 1 (TJP1). TJPl is located on a cytoplasmic membrane surface of intercellular tight junctions and may be involved m signal transduction at cell-cell junctions.

303710

BAA03274.1

P39447

194,742 Da

Adherens Junction Pathway (83267); Adherens Junction Pathway (481); Gap Junction Pathway (83269); Gap Junction Pathway (483); Gap Junction Trafficking And Regulation Pathway (927299); Membrane Trafficking Pathway (927292); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); Regulation Of Gap Junction Activity Pathway (927308); Salmonella Infection Pathway (375174); Salmonella Infection Pathway (375149)

Function: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells . By similarity. Subunit structure: Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with CGN/cingulin, CALM, CXADR, GJD3, HSPA4 and UBN1. Interacts (via ZU5 domain) with CDC42BPB. Interacts (via PDZ domain) with GJA1 . By similarity. Interacts with OCLN, claudins, GJA12 and KIRREL1. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts (via ZU5 domain) with MYZAP. Interacts (via PDZ domains) with ANKRD2 . By similarity. Ref.3 Ref.4 Ref.5 Ref.12. Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side . By similarity. Cell junction tight junction . By similarity. Cell junction gap junction. Cytoplasm myofibril sarcomere I band. Note: Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells . By similarity. Ref.12. Domain: The second PDZ domain mediates interaction with GJA12. Ref.4. Post-translational modification: Phosphorylated. Dephosphorylated by PTPRJ . By similarity. Sequence similarities: Belongs to the MAGUK family.Contains 1 guanylate kinase-like domain.Contains 3 PDZ (DHR) domains.Contains 1 SH3 domain.Contains 1 ZU5 domain.

0.6 mg

410 USD