antibody

Monocloncal Antibody NITEGE (Clone BC13)

MBS442004

0.1 mg

405 USD

monoclonal

mouse

nonconjugated

BC-13

western blot, ELISA, immunohistochemistry

Antibody

Monocloncal Antibody NITEGE (Clone BC13)

Aggrecan, C-terminal neoepitope NITEGE

Aggrecan Antibody, C-terminal neoepitope NITEGE

Monoclonal

IgG1

BC-13

Mouse

Human, Cow, Dog, Horse, Pig

Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated C-terminal neoepitope . TEGE after cleavage between amino acids . EGE373 and 374ARG. within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).

Affinity purified on protein G

Liquid, 1 mL/vial

0.1 mg/mL

The antibody is stable until the expiry date given on the label if stored at -20 degree C. Repeated freezing and thawing should be avoided. Aliquoting is recommended.

Western Blotting, ELISA, IHC.

Western Blotting: . Suggested dilution: 1:100. Detects a a band of approximately 60-70kDa.

Immunogen: NITEGE synthetic peptide conjugate. Myeloma: x63-Ag8.653. Light Chain Type: kappa

Technical Notes: Samples are usually deglycosylated using 0.01 Units chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry (1, 2). Storage Buffer: PBS, no preservatives.

Antibodies to Aggrecan

Aggrecan monoclonal antibody to C-terminal neoepitope NITEGE (mouse, clone BC-13). This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue. Overview: Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.

0.1 mg

405 USD