antibody

PPARgamma (C15) Antibody

MBS440118

0.2 mg

240 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, chicken, cow

Antibody

PPARgamma (C15) Antibody

PPARgamma

peroxisome proliferator-activated receptor gamma isoform 2; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3

PPARgamma

PPARG; PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma; NR1C3; PPAR-gamma

PPARG_HUMAN

Polyclonal

Rabbit

Mouse, rat, human, hamster, chicken, dog, bovine, pig

505

Recognize mouse, rat, human, hamster, chicken, dog, bovine, and pig PPARgamma1 and PPARgamma2 by western blotting.

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

0.200 mg/ml

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB)

Recommended for western blotting, starting dilution 1:400.

Origin: PPARgamms (C15) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping near the carboxy terminus of human PPARgamma.

Immunogen: A synthetic peptide mapping near the carboxy terminus of human PPARgamma.

Peroxisome proliferator-activated receptors (PPAR) are members of the steroid nuclear receptor superfamily (1). The mammalian PPARs include three subtypes PPARalpha, PPARbeta, and PPARgamma. PPARs have been found to regulate fatty acid oxidation, fat cell development, lipoprotein metabolism, and glucose homeostasis (2). Recent studies have found that PPARs can affect the pathogenesis and development of tumors (3). PPARgamma and PPARbeta have been specifically implicated in tumorigenesis (3). PPARalpha is the focus of research studying its function in fatty acid beta-oxidation, lipid metabolism, and vascular inflammation (4). PPARbeta also plays a key role in the activation of keratinocytes during the inflammatory reaction associated with a skin injury (5). Of the three subtypes of PPARs, PPARgamma is the most widely studied. PPARgamma has been implicated in various human chronic diseases such as diabetes mellitus, atherosclerosis, rheumatoid arthritis, inflammatory bowel disease, and Alzheimer¿s disease (6).

1.Bocos C, Gottlicher M, Gearing K, Banner C, Enmark E, Teboul M, Crickmore A, and Gustafsson JA. 1995. Fatty acid activation of peroxisome proliferator-activated receptor (PPAR). J Steroid Biochem Mol Biol 53(1-6):467-473.2.Li AC, and Glass CK. 2004. PPAR and LXR-dependent pathways controlling lipid metabolism and development of atherosclerosis. J Lipid Res.3.Nahle Z. 2004. PPAR trilogy from metabolism to cancer. Curr Opin Clin Nutr Metab Care. 7(4):397-402.4.van Raalte DH, Li M, Pritchard PH, Wasan KM. 2004. Peroxisome proliferator-activated receptor (PPAR)-alpha: a pharmacological target with a promising future. Pharm Res. 21(9): 1531-1538.5.Tan NS, Michalik L, Desvergne B, Wahli W. 2003. Peroxisome proliferator-activated receptor (PPAR)-beta as a target for wound healing drugs: what is possible? Am J Clin Dermatol. 4(8): 523-530.6.Takano H, Hasegawa H, Zou Y, Komuro I. 2004. Pleiotropic actions of PPAR gamma activators thiazolidinediones in cardiovascular diseases. Curr Pharm Des. 10(22): 2779-2786.

20336229

NP_056953.2

NM_015869.4

P37231

21,580 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Developmental Biology Pathway (477129); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Huntington's Disease Pathway (83100)

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described. Protein type: Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol. Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; retinoid X receptor binding; arachidonic acid binding; protein binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity. Biological Process: negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; heart development; cell maturation; rhythmic process; lipid homeostasis; glucose homeostasis; response to lipid; response to caffeine; response to vitamin A; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to mechanical stimulus; lipoprotein transport; response to estrogen stimulus; brown fat cell differentiation; positive regulation of fat cell differentiation; fatty acid oxidation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; positive regulation of phagocytosis, engulfment; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; white fat cell differentiation; positive regulation of fatty acid oxidation; innate immune response; gene expression; lipid metabolic process; response to cold; negative regulation of cell growth. Disease: Obesity; Lipodystrophy, Familial Partial, Type 3; Carotid Intimal Medial Thickness 1; Diabetes Mellitus, Noninsulin-dependent

0.2 mg

240 USD